FAQ
How do I apply molecular weight markers onto my 2-D gel?
Apply molecular weight marker proteins to a sample application piece in a volume of 15-20 µl, then cover the piece with 50 µl of agarose sealing solution. Pick up the application piece with forceps and place next to one end of the IPG strip, then seal both the IPG strip and the MW markers with agarose sealing solution. The markers should contain 0.2-1.0 µg of each component for Coomassie blue staining or about 10-50 ng of each component for silver staining.
How do I apply molecular weight markers onto my 2-D gel?
Apply molecular weight marker proteins to a sample application piece in a volume of 15-20 µl, then cover the piece with 50 µl of agarose sealing solution. Pick up the application piece with forceps and place next to one end of the IPG strip, then seal both the IPG strip and the MW markers with agarose sealing solution. The markers should contain 0.2-1.0 µg of each component for Coomassie blue staining or about 10-50 ng of each component for silver staining.