FAQ
Purifying SBP-tagged proteins using Streptavidin Sepharose High Performance or HiTrap Streptavidin HP has not been tested in our labs. It should however be possible. Information on buffer systems used can be found in the following paper:
Keefe, A. D. et al. One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag. Protein Expr. Purif. 23, 440–446 (2001).
There is not a cleaning procedure for Streptavidin Sepharose. Due to the harsh conditions for elution, there is a big risk of destroying the streptavidin immobilized to the gel. The products strongly and specifically bind biotinylated biomolecules. Streptavidin has a very high affinity for biotin (Kd = 4 x 10-14 M) and the binding is irreversible. The only way to be able to reuse your column is to work with iminobiotinylated molecules.