FAQ
How can I check whether my ECL Advance reagent is working properly?
You can perform the "blue-light test." Simply combine equal volumes of Detection Reagents 1 and 2 in a microcentrifuge tube. Mix, and add several µL of an HRP-conjugated antibody that is known to be functional to the tube. Mix well and turn out the lights in a dark room. Functional reagents will begin to glow bluish-white fairly rapidly.
What blocking agents are recommended for ECL Advance Western Blotting Detection Reagents?
We only recommend using the ECL Advance Blocking Reagent supplied with the ECL Advance Detection Reagents. Other blocking agents have been shown to give a high background.