Cellbag BioClear 10 (Perfusion Version)

ID	Product Name
28412278	Cellbag BioClear 10
28412286	Cellbag BioClear 10
28412269	Cellbag BioClear 10
28412268	Cellbag BioClear 10
28412304	Cellbag BioClear 10
28412411	Cellbag BioClear 10
28412399	Cellbag BioClear 10 (Oxywell Version)
28412279	Cellbag BioClear 10 (Oxywell Version)
28412270	Cellbag BioClear 10 (Oxywell Version)
28412287	Cellbag BioClear 10 (Oxywell Version)
28412305	Cellbag BioClear 10 (Oxywell Version)
28412301	Cellbag BioClear 10 (Oxywell Version)
28412359	Cellbag BioClear 10 (Perfusion Version)
28412354	Cellbag BioClear 10 (Perfusion Version)
28412342	Cellbag BioClear 10 (Perfusion Version)
29176218	Cellbag BioClear 10 (Perfusion Version)
28412271	Cellbag BioClear 10 (Screw Cap Port)
28412280	Cellbag BioClear 10 (Screw Cap Port)
28412288	Cellbag BioClear 10 (Screw Cap Port)
28412397	Cellbag BioClear 10, (Oxywell Version)
28413016	Cellbag BioClear 10, BC10, DO, RM
28413019	Cellbag BioClear 10, BC10, DO, RM
28413018	Cellbag BioClear 10, BC10, DO, RM
28413020	Cellbag BioClear 10, BC10, DO, RM
28413017	Cellbag BioClear 10, BC10, DO, RM
28984091	Cellbag BioClear 10, BC10, pHOPT, DO
28984092	Cellbag BioClear 10, BC10, pHOPT, DO
28984079	Cellbag BioClear 10, BC10, pHOPT, DO
28984074	Cellbag BioClear 10, BC10, pHOPT, DO
28984089	Cellbag BioClear 10, BC10, pHOPT, DO
28984080	Cellbag BioClear 10, BC10, pHOPT, DO
28984090	Cellbag BioClear 10, BC10, pHOPT, DO
29018907	Cellbag BioClear 10, DOOPT II and pHOPT
29018906	Cellbag BioClear 10, DOOPT II and pHOPT
29015216	Cellbag BioClear 10, DOOPT II and pHOPT
29015214	Cellbag BioClear 10, DOOPT II and pHOPT
29015215	Cellbag BioClear 10, DOOPT II and pHOPT
29015212	Cellbag BioClear 10, DOOPT II and pHOPT
29015312	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015315	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015213	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015308	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015444	Cellbag BioClear 10, DOOPT II, pHOPT and Perfusion (Thick Filter)
29015309	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29015307	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29015313	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29012460	Cellbag BioClear 10, pHOPT and perfusion
29012355	Cellbag BioClear 10, pHOPT and perfusion
29012360	Cellbag BioClear 10, pHOPT and perfusion
29012470	Cellbag BioClear 10, pHOPT and Perfusion (Standard Filter)
29012353	Cellbag BioClear 10, pHOPT and screwcap
29012357	Cellbag BioClear 10, pHOPT and screwcap

Troubleshooting
Accessories

Accessories

Accessories for Cellbag perfusion version CB0010L10-04 and Cellbag, 10L, BC11, Perf, DO CB0010L11-04

Port No.	Description	Associated products
1	3/16 in × 3/8 in × 2 in silicone, needleless sampling	Syringe with Luer male connection.
2	1/8 in × 1/4 in × 39 in C-Flex®, Luer female	Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001).
3	Oxywell2, for DOOPT probe	DOOPT-Probe 28411672 and Oxywell2 28412451
4	Y-connection attached to perfusion filter	Perfusion controller module for use with cellbag 2L/P and cellbag 10L/P 28987584
5	3/16 in × 3/8 in × 2 in silicone, Luer female	Connector Luer male and tubing i.d. 3/16 in
6	Air inlet filter	Tubing to connect inlet filter to system supplied with system.
7	Air outlet filter, check valve	Electrical heater for cellbag vent filter 28411639, check valve WV050087. Tubing to connect outlet filter to system supplied with system.
8	3/16 in × 3/8 in × 2 in silicone, Luer female	Connector Luer male and tubing i.d. 3/16 in
Int.	Perfusion filter

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#	Product Name	Product Code	Price
4	PERFCONT 2E	28987584	15,000.00 USD	Add to cart
7	Electric Exhaust Filter Heater	28411639	548.00 USD	Add to cart

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Troubleshooting for Cellbags

The Cellbag appears to be overinflated

Possible cause	Suggested remedy
Too high airflow	Check that the airflow to the bioreactor does not exceed 0.5 Lpm. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)
Faulty relief valve	Check that air is passing out of the pressure relief valve: attach a short length of tubing to the exhaust vent and immerse the tubing in water to a 1 cm depth. Bubbles should appear indicating flow. If no flow is observed, remove the pressure relief valve. The outlet filter may be plugged and removing the pressure relief valve may allow continued operation. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)
Faulty Cellbag	If the Cellbag continues to overinflate, transfer the contents to another Cellbag. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Possible cause

Suggested remedy

Too high airflow

Check that the airflow to the bioreactor does not exceed 0.5 Lpm.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Faulty relief valve

Check that air is passing out of the pressure relief valve: attach a short length of tubing to the exhaust vent and immerse the tubing in water to a 1 cm depth. Bubbles should appear indicating flow. If no flow is observed, remove the pressure relief valve. The outlet filter may be plugged and removing the pressure relief valve may allow continued operation.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Faulty Cellbag

If the Cellbag continues to overinflate, transfer the contents to another Cellbag.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

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The Cellbag appears to be underinflated. (An underinflated Cellbag will generate excessive foam and poor mixing.)

Possible cause	Suggested remedy
Too low airflow	Check that there is sufficient air flow to the inlet filter. (An underinflated Cellbag will generate excessive foam and poor mixing.)
Inlet air supply wrongly connected	Check that you have connected the inlet air supply line to the inlet filter.
Missing pressure relief valve	Check that the pressure relief valve is present on the exhaust filter.
Obstructed flow paths	Check that air inlet and out flow paths are unobstructed.
Clogged exhaust filter	If the exhaust filter is clogged then transfer the contents to another Cellbag.

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Excessive foaming in the Cellbag. Some amount of foam is typical. Foam should not cover more than 50% of the surface area.

Possible cause	Suggested remedy
Poorly inflated Cellbag	Check that the Cellbag is rigidly inflated. A poorly inflated bag will foam rapidly.
Too high rocking rate	If foam is still excessive after a few hours of operation, reduce the rocking rate. Make sure that the new rocking rate provides a sufficient dissolved oxygen concentration.
Too big rock angle	Reduce the rock angle. The bioreactor base units are factory set to ±6° from the horizontal. This angle has been found optimal for most cell lines.

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