Accessories
Port No. | Description | Associated products |
---|---|---|
1 | Oxywell2, for DOOPT probe | DOOPT-Probe 28411672 and Oxywell2 28412451 |
2 | 3/8 in × 5/18 in × 78 in C-Flex®, MPC male | Connector MPC female and tubing i.d. 3/8 in |
3 | 3/16 in × 3/8 in × 2 in silicone, Luer female | Connector Luer male and tubing i.d. 3/16 in |
4 | 3/16 in × 3/8 in × 2 in silicone, needleless sampling | Syringe with Luer male connection |
5 | 1/4 in × 7/16 in × 78 in C-Flex®, MPC male | Connector MPC female and tubing i.d.1/4 in |
6 | 1/8 in × 1/4 in × 78 in C-Flex®, Luer female | Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001) |
7 | Tempwell for RTD probe | RTD temperature probe 28411667 |
8 | Air inlet filter | Tubing to connect inlet filter to system supplied with system |
9 | Air outlet filter, check valve |
Electrical heater for cellbag vent filter 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system |
10 | Air outlet filter, check valve |
Electrical heaters for cellbag vent filters 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system |
11 | 3/8 in × 5/18 in × 78 in C-Flex®, MPC male, silicon diptube | Connector MPC female and tubing i.d. 3/8 in |
# | Product Name | Product Code | Price | |
---|---|---|---|---|
9 | Electric Exhaust Filter Heater | 28411639 | 548.00 USD |
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10 | Electric Exhaust Filter Heater | 28411639 | 548.00 USD |
Add to cart
|
Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Troubleshooting for Wave Bioreactor System 200 and Wave Bioreactor System 500
Possible cause | Suggested remedy |
---|---|
The outlet filter is closed off or blocked. |
Make sure that the outlet filter is not closed off or blocked. Try opening the spare (middle) filter to depressurize the Cellbag. You can connect this filter to the Exhaust pump and continue operations. If foam is clogging the outlet filter, reduce the rocking speed or the rock angle. (The Cellbag should be inflated so that it requires force to squeeze it 2 inches H2O ± 0.2 inches for WAVE Bioreactor 200 and 3 inches H2O ± 0.2 inches for WAVE Bioreactor 500/1000). However, it should not be so pressurized that creases form near the attachment points. The inlet airflow will shut off automatically (PV=0) if the pressure exceeds pressure setpoint +1 inch H2O gauge)
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If your are using an Exhaust pump - the Exhaust pump is disconnected or turned off |
Make sure that the correct Exhaust pump is connected to the respective outlet filter. Check to see that the Exhaust pump is running.
(The Cellbag should be inflated so that it requires force to squeeze it 2 inches H2O ± 0.2 inches for WAVE Bioreactor 200 and 3 inches H2O ± 0.2 inches for WAVE Bioreactor 500/1000). However, it should not be so pressurized that creases form near the attachment points. The inlet airflow will shut off automatically (PV=0) if the pressure exceeds pressure setpoint +1 inch H2O gauge |
Possible cause | Suggested remedy |
---|---|
Too low airflow |
Check that there is sufficient air flow to the inlet filter.
(An underinflated Cellbag will generate excessive foam and poor mixing. |
Clogged inlet filter |
If the inlet filter is clogged, use the spare filter as an inlet filter.
(An underinflated Cellbag will generate excessive foam and poor mixing. |
Possible cause | Suggested remedy |
---|---|
Poorly inflated Cellbag |
Check that the Cellbag is rigidly inflated. A poorly inflated Cellbag will foam rapidly.
(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.) |
Too high rocking rate |
In many cases foam will subside after a few hours of operation. If foam is still excessive, reduce the rocking rate. Check that this rocking rate still provides a sufficient dissolved oxygen concentration.
(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.) |
Too big rock angle |
Reduce the rock angle. Reducing the angle will reduce foam while being able to maintain a higher rock rate. The WAVE Bioreactor base units are factory set to ±5° from the horizontal. This angle has been found to be optimal for most cell lines.
(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.) |