ReadyToProcess MabSelect SuRe 20 L (359/200)
These ReadyToProcess chromatography columns are prepacked with MabSelect SuRe chromatography resin and are excellent for capture of monoclonal antibodies (mAbs) at process scale. ReadyToProcess columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use.
FAQ
Same procedures as for packed conventional process columns. Equilibrate and store packed columns according to the instructions for the medium in the ReadyToProcess column user manual.
Same procedures as for packed conventional process columns. Please read the Application note: “Efficiency test of ReadyToProcess columns”, available in the Related Literature tab.
Product documentation—a user manual, including a certificate of conformance, is provided. The certificate of conformance shows conformance with 21CFR177, USP Class VI, and animal-free origin (or EMEA/410/01) for each wetted part material as well as traceability of the material. The product documentation contains a certificate of analysis showing packing performance as well as endotoxin and microbiology test results for the delivered column. The user manual is distributed with each column and the CoA can be downloaded from gelifesciences.com/certificates.
Validation guides—gives access to product information of the ReadyToProcess column including stability, validation, and quality as well as a brief description of the production process.
RSF—current files include additional information on each BioProcess chromatography resin. RSF addenda and RSF are available at gelifesciences.com/rsf
Rigorous transport studies have shown that there is no change in column performance after shipping. Beta testers confirm splendid performance. Shipping and drop tests are included in Regulatory Support File.
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Possible causes |
Suggested remedy |
|---|---|
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Column is clogged. |
1. Perform regeneration of the column |
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Bent tubing. |
Check that the flow path is not restricted. |
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Buffer viscosity too high. |
Check the viscosity of all buffers. Viscosity is a function of temperature. (Lower temperature gives higher viscosity.) Let low-temperature buffer reach operating temperature before starting the run. |
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Microbial growth in buffers. The buffer normally become opalescent due to microbial growth. |
Check buffers, especially those with phosphate, for microbial growth. Replace with fresh buffer if necessary. |
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Sample and collection vessels at different levels. |
Adjust the vessels to approximately the same level. |
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The prefilter might be blocked. |
Check the prefilter. |
| 1 L column | 2.5 L column | 5 L column | 10 L column | 20 L column | 32 L column |
|---|---|---|---|---|---|
| ~3 kg | ~6 kg | ~13 kg | ~25 kg | ~55 kg | ~96 kg* |
*Wheels for convenient handling.
Accessories
1. Assemble the Normal flow filter (NFF) inlet to the flow kit.
2. Evacuate air by filling NFF with liquid. Use the system to pump liquid through the system to the NFF.
3. Assemble the NFF to the column inlet. Extra tubing may be needed to prolong the flow kit. Please make sure the tubing is not folded and does not contain any air.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| TC clamp kit, 25 mm TC | 28404336 | 302.00 USD |
Add to cart
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| TC Clamp kit TC25/SS | 28404338 | 148.09 USD |
Add to cart
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| TC-gasket kit 25 mm i.d. 6.5 mm | 28966737 | 57.00 USD |
Add to cart
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| TC-Gasket 25 mm i.d. 15 mm | 44549288 | 209.00 USD |
Add to cart
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1. Assemble the Normal flow filter (NFF) inlet to the flow kit.
2. Evacuate air by filling NFF with liquid. Use the system to pump liquid through the system to the NFF.
3. Assemble the NFF to the column inlet. Extra tubing may be needed to prolong the flow kit. Please make sure the tubing is not folded and does not contain any air.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| TC clamp kit, 25 mm TC | 28404336 | 302.00 USD |
Add to cart
|
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| TC Clamp kit TC25/SS | 28404338 | 148.09 USD |
Add to cart
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| Clamp, 50 mm TC | 28938191 | 265.00 USD |
Add to cart
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| Gasket 51 mm | 28966886 | 91.28 USD |
Add to cart
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| TC-Gasket 25 mm i.d. 15 mm | 44549288 | 209.00 USD |
Add to cart
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| Gasket 50 mm (1.5") TC i.d. 38 mm, silicone | 56410996 | 92.12 USD |
Add to cart
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