Consumables
# | Product Name | Product Code | Price | |
---|---|---|---|---|
Gel Filtration LMW Calibration Kit | 28403841 | 835.37 USD |
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Gel Filtration HMW Calibration Kit | 28403842 | 835.37 USD |
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Accessories
# | Product Name | Product Code | Price | |
---|---|---|---|---|
Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 123.16 USD |
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Tricorn Storage/Shipping Device | 18117643 | 139.40 USD |
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Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Superdex 200 Increase 5/150 GL
Issues related to Superdex 200 Increase 5/150 GL
Possible cause | Suggested remedy |
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Increased back-pressure over the column and/or loss of resolution. |
Confirm that the column is the cause (see below). If so, clean it according to the procedure described in the section Cleaning in place (CIP). To confirm that the high back-pressure in the system is caused by the column, disconnect one piece of equipment at a time (starting at the fraction collector) with the pumps running. Check the pressure reading after each piece has been disconnected to determine the source of the back-pressure. |
Possible cause | Suggested remedy |
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Air in the column |
Note that small amounts of air will normally not affect the performance of the column. Run 3 to 4 column volumes (CV) of well de-gassed eluent in an upflow direction at a flow rate of 0.2 ml/min (5/150) or 0.5 ml/min (10/300) at room temperature. |
Possible cause | Suggested remedy |
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Space between gel bed and adapter. |
Turn down the adapter to the gel bed. Perform a Column performance control. |