Fraction Collector F9-C

ID	Product Name
29027743	Fraction Collector F9-C

Troubleshooting
Accessories

Accessories

Fraction collector F9-C

Front view

Part	Description
1	Fractionation indicator Symbol indicating that fractionation is ongoing. Do not open the door while the indicator is lit.
2	Door
3	Window
4	Door handle
5	Tubing connector for outlet valve tubing
6	Vents

Rear view

Part	Description
1	Vents
2	UniNet-9 D-type connector (for communication and power supply)
3	Waste tube

Interior

Part	Description
1	Fractionation arm guide rail
2	Fractionation arm main rail
3	Lamp
4	Tubing guide
5	Tubing connection
6	Dispenser head
7	Fractionation arm
8	Tubing guide
9	Height exclusion bar
10	Waste funnel
11	Waste tube
12	Tray catch
13	Waste groove, in case of overflow
14	Tray guides

Dispenser head

Part	Description
1	Dispenser head
2	Nozzle
3	Dispenser head cover
4	Accumulator (back part of Dispenser head)
5	Drop sync sensor
6	Type code reader

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Product Name	Product Code	Price
UNIPLATE Collection and Analysis Microplate, 24-well, 10 ml, natural polypropylene, round well bottom	28420867	343.00 USD	Add to cart
UNIPLATE Collection and Analysis Microplate, 96-well, 2 ml, natural polypropylene, round well bottom	28420869	273.00 USD	Add to cart
UNIPLATE Collection and Analysis Microplate, 48-well, 5 ml, natural polypropylene, rectangular well, flat bottom	28420874	350.00 USD	Add to cart
Cassette tray	28954209	310.00 USD	Add to cart
Cassette for fraction collection in deep-well plate	28954212	194.58 USD	Add to cart
Cassette for 50 ml tubes	28956402	197.68 USD	Add to cart
Cassette, for 15 ml tubes	28956404	197.68 USD	Add to cart
Cassette for fraction collection in 8 mL tubes	28956425	198.72 USD	Add to cart
Cassette, for 3 ml tubes	28956427	198.72 USD	Add to cart
Rack, for 50 ml tubes	28980319	774.00 USD	Add to cart
Rack for fraction collection in 250 mL bottles	28981873	1,660.00 USD	Add to cart
Fraction Collector F9-C	29027743	12,500.00 USD	Add to cart
Cable 2.5m UniNet-9 D-type	29032425	140.76 USD	Add to cart

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Fraction collector F9-C

Issues related to Fraction collector F9-C

The fraction collector cannot be found by the instrument

Possible cause	Suggested remedy
The cable between the fraction collector and the ÄKTA pure instrument is not connected	Generate a System error report and contact Service.
A fuse in the instrument ICU is broken	The ICU needs to be changed. Generate a System error report and contact Service.

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Fraction collector arm is blocked or internal fault in the fraction collector

Possible cause	Suggested remedy
Obstruction inside the fraction collector	Switch off the instrument and check for obstruction inside the fraction collector. Try to move the fractionation arm by hand. Switch on the instrument. If this error is recurrent, generate a System error report and contact Service.

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The accumulator is jammed or there is an internal error in the instrument

Possible cause	Suggested remedy
Salt crystals or protein residuals block the accumulator	Restart the instrument and perform an accumulator wash.
Mechanical error	If this error is recurrent, generate a System error report and contact Service.

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Fraction collection tube or well is overfilled and fractionation movements are lost

Possible cause	Suggested remedy
Too many commands are pending in the fraction collector	The reason could be that too many fraction collector instructions have been sent. Wait for a while and try again.

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The fraction collector failed to detect the code on the Cassette

Possible cause	Suggested remedy
Cassette tray or tube rack is inserted in the wrong direction	Take it out and insert it in the correct direction.
Unclean Cassette type code reader	Clean the dispenser head and its four diode windows using a cloth and a mild cleaning agent or 20% ethanol. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information.
Unclean Cassette type codes	Clean the Cassette type codes. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. If this error is recurrent, set the Fraction collector configuration manually in UNICORN. In *System Control, select System:Settings. Navigate to Fraction collector:Cassette configuration* and select *Manual*.

Possible cause

Suggested remedy

Cassette tray or tube rack is inserted in the wrong direction

Take it out and insert it in the correct direction.

Unclean Cassette type code reader

Clean the dispenser head and its four diode windows using a cloth and a mild cleaning agent or 20% ethanol. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information.

Unclean Cassette type codes

Clean the Cassette type codes. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information.

If this error is recurrent, set the Fraction collector configuration manually in UNICORN. In System Control, select System:Settings. Navigate to Fraction collector:Cassette configuration and select Manual.

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The Cassette tray is loose in the fraction collector

Possible cause	Suggested remedy
The Tray guides in the fractionation chamber are missing	Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information.
The Tray catch is broken	Contact service.

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Calibration of the Drop sync sensor failed

Possible cause	Suggested remedy
Unclean Drop sync sensor diode windows	Clean the Drop sync sensor diode windows. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for location of the Drop sync sensor diode windows and cleaning instructions. If this error is recurrent, generate a System error report and contact Service.

Possible cause

Suggested remedy

Unclean Drop sync sensor diode windows

Clean the Drop sync sensor diode windows. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for location of the Drop sync sensor diode windows and cleaning instructions.

If this error is recurrent, generate a System error report and contact Service.

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Drop sync does not work

Possible cause	Suggested remedy
Liquid spatter in the fraction collector	Change to another type of tubes or deepwell plates or collect fraction volumes of maximum two thirds of the volume of the tubes. Clean the diode windows of the drop sync sensor more frequently than once a week, using a cloth and a mild cleaning agent or 20% ethanol.

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The Dispenser head failed to detect a drop

Possible cause	Suggested remedy
Air in the flow path	If this error is recurrent, generate a System error report and contact Service. Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook.
Unclean Drop sync sensor diode windows	Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. If this error is recurrent, generate a System error report and contact Service.
Too high flow rate	Decrease the flow rate.

Possible cause

Suggested remedy

Air in the flow path

If this error is recurrent, generate a System error report and contact Service.

Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook.

Unclean Drop sync sensor diode windows

Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. If this error is recurrent, generate a System error report and contact Service.

Too high flow rate

Decrease the flow rate.

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The Dispenser head failed to detect the flow properly and has switched to tube change with reduced accumulator functionality

Possible cause	Suggested remedy
Air in the flow path	Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service.
Unclean diode windows in the Drop sync sensor	Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service.

Possible cause

Suggested remedy

Air in the flow path

Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook.

If this error is recurrent, generate a System error report and contact Service.

Unclean diode windows in the Drop sync sensor

Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service.

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The Dispenser head failed to detect a drop and has switched to tube change without Drop sync

Possible cause	Suggested remedy
Too high flow rate	Decrease the flow rate.
Air in the flow path	Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service.
Unclean diode windows in the Drop sync sensor	Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service.

Possible cause

Suggested remedy

Too high flow rate

Decrease the flow rate.

Air in the flow path

Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook.

If this error is recurrent, generate a System error report and contact Service.

Unclean diode windows in the Drop sync sensor

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Tubes do not fit in the Cassette

Possible cause	Suggested remedy
Wrong tube dimensions are used	Check that the used tubes have the right dimensions. See Fraction collector tubes, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook.
QuickRelease function is worn out	Order a new Cassette. See Chapter 11 in the ÄKTA pure System Handbook for ordering information.

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Deep well plate does not fit in the Cassette

Possible cause	Suggested remedy
Unsupported deep well plate model	Check that the deep well plates are supported. See Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook.

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The Cassette does not fit the Cassette tray

Possible cause	Suggested remedy
The Cassette is turned in the wrong direction	See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook for information of how to place the Cassettes.
Objects or dirt under the Cassette	Remove the object or dirt.

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Cassette tray in wrong position

Possible cause	Suggested remedy
The Tray guides in the fractionation chamber are missing	Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.
The Cassette tray is facing the wrong direction	Make sure that the front of the tray (marked with the GE-logotype) is facing outwards. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook.
Dirt under the Cassette tray	Remove the dirt.

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The loaded Cassette tray cannot be inserted into the fractionation collector

Possible cause	Suggested remedy
The Cassette tray is facing the wrong direction	Check that the GE-logotype is facing outwards when the tray is inserted into the fraction collector.
Some of the tubes or plates are incorrectly placed in the Cassettes	Check that all tubes and plates are correctly inserted in the Cassettes. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook.
Some of the tubes or plates have the wrong dimensions	Check that the deep well plates and the tubes used are of the right type. See Fraction collector tubes and Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook for information about supported tubes and plates.

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Quick scan or Full scan does not work

Possible cause	Suggested remedy
The Tray guides in the fractionation chamber are missing	Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.
The Cassette tray is facing the wrong direction	Check that the GE-logotype is facing outwards.
The Cassette type codes are unclean	Clean the the Cassette type codes.
The QuickRelease of a Cassette is in open position	Close the Cassette. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook.
The Cassette code reader diode window is unclean	Clean the Cassette code reader diode window. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.
The automatic scanning is turned off in UNICORN	Make sure that the automatic scanning is turned on in UNICORN software. In *System Control, select System:Settings.* Navigate to *Fraction collector:Cassette configuration* and select *Automatic*.
Wells in deep well plates are prefilled to a volume above 25% of the total well volume	Full scan will not work with prefilled wells during these conditions.
Hardware error	Generate a System error report and contact Service.

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The interior of the fraction collector is dark

Possible cause	Suggested remedy
The light has been turned off in UNICORN	Turn on the light in UNICORN. In *System Control,* select *System: Settings. Navigate to Fraction collector:Fraction collector lamp* and select *Lamps On*.
The lamp is broken	Contact Service.

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The waste or the interior of the fraction collector is flooded

Possible cause	Suggested remedy
The waste tubing is positioned so that the flow is obstructed	Untwist the waste tubing.
The waste container is placed in a position higher than the waste outlet	Place the waste container in a position lower than the waste outlet.
The waste tubing is blocked	Clean or replace the waste tubing.

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The liquid leaving the nozzle does not strike the waste funnel

Possible cause	Suggested remedy
Check the position of the waste funnel	Refit the waste funnel.

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The tubing in the fraction collector is blocked

Possible cause	Suggested remedy
Salt residuals in the tubing	Perform a *Fraction collector wash, see Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook*. If persistent, contact Service.

Possible cause

Suggested remedy

Salt residuals in the tubing

Perform a Fraction collector wash, see Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.

If persistent, contact Service.

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The fraction volume found in the tubes or wells are smaller than expected

Possible cause	Suggested remedy
Leakage on the wet side of the instrument	Localize the leakage and take care of the leakage, for example by tightening connectors.
Air in pumps	Purge pumps. See Section 6.4.1 System pumps in the ÄKTA pure System Handbook.
Bad pump function	See troubleshooting of pumps in Section 9.5 in the ÄKTA pure System Handbook.
Leakage inside the Frac chamber	Contact Service.

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Liquid on the floor of the fraction collector

Possible cause	Suggested remedy
The fingertight connector on the Dispenser head is not tight enough	Tighten the connector. Replace the connector if the leakage is persistent.
The waste tubing or the waste funnel is blocked.	Make sure that there is no blockage or clogging and that the waste tubing not is bent.
Leakage in the Frac arm	Contact service.

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Spillage by the fraction collector during fractionation

Possible cause	Suggested remedy
Unclean diode windows in the Drop sync sensor or unclean nozzle	Clean the Drop sync diode windows and the nozzle. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. Use the Fraction collector cleaning position.
Too high flow rate during usage of Drop sync	Use a flow rate below 2 ml/min.
One or more Cassettes have empty positions	Make sure that all Cassette positions contain tubes or plates.
The tubes are flooded	Make sure that the fraction volume is adapted to the tube volume. Fraction tubes have not been replaced by empty tubes when the Fraction collector was opened.

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It is not possible to fractionate

Possible cause	Suggested remedy
The door of the fraction collector is not properly closed	Close the door.

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The fraction collector fractionates in the wrong well or tube

Possible cause	Suggested remedy
Quick scan has not detected the correct Cassette	Clean the Cassette type code of the Cassette. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.
Unsupported deep well plate is used.	Make sure that approved deep well plates are used. See Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook.
Wells in deep well plates are prefilled to a volume above 25% of the total well volume	Full scan will not work with prefilled wells during these conditions.
The deep well plate is not correctly positioned in the Cassette	See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook for information of how to place the deep well plate.
Dirt on the nozzle or Drop sync sensor diode windows. Dirt may effect where the drops fall.	Clean nozzle and Drop sync sensor diode windows. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook.

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Error message when the door of the fraction collector is opened during a run

Possible cause	Suggested remedy
Some parts in a method require that the door of the fraction collector is closed	Do not open the door when the Fractionation indicator is lit.

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The instrument does not fractionate via the Outlet valve ports

Possible cause	Suggested remedy
The Detector - Outlet valve delay volume has been set to zero.	In *System Control, select System:Settings:Fraction collections:Delay volumes*. Correct the Detector - Outlet valve delay volume.

Possible cause

Suggested remedy

The Detector - Outlet valve delay volume has been set to zero.

In System Control, select System:Settings:Fraction collections:Delay volumes.
Correct the Detector - Outlet valve delay volume.

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When starting a method run during a manual run, the fraction collector generates an error message indicating that the wrong Cassettes are in place, even though the correct Cassettes are present in the Fraction collector.

Possible cause	Suggested remedy
	Action: End a manual run before starting a method run.

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High pressure alarm when collecting fractions with the fraction collector

Possible cause	Suggested remedy
	Action: Decrease the flow rate or use Outlet valve fractionation, or replace the following tubing with tubing of larger inner diameter (i.d.) - the tubing between the outlet valve and the fraction collector and - the internal tubing of the fraction collector. *Note:* Update the delay volume. In the System Control software module, select System:Settings:Tubing and Delay volumes and update the value for Delay volume: Monitor to frac.

Possible cause

Suggested remedy

Action:

Decrease the flow rate or use Outlet valve fractionation, or
replace the following tubing with tubing of larger inner diameter
(i.d.)

- the tubing between the outlet valve and the fraction collector
and

- the internal tubing of the fraction collector.

Note:
Update the delay volume. In the System Control software module, select System:Settings:Tubing and Delay volumes and update the value for Delay volume: Monitor to frac.

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Spillage in Fraction collector when the Frac arm is moving from Cassette placed in position 1 and 2 to waste

Possible cause	Suggested remedy
	Action: If possible, place the Cassettes close to waste, i.e., position 5 or 6 during fractionation.

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The fractionation starts in the first row again

Possible cause	Suggested remedy
The door of the fraction collector has been opened between two runs. When the instrument is in state Ready and the door is opened and closed, the Fraction collector content is reset.	If fractionation will continue after the last fraction of the first run, do not open the door of the fraction collector between two runs.

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Spillage between fractions

Possible cause	Suggested remedy
The combination of high flow rates and liquids with low surface tension might lead to spillage in the Fraction collector.	If possible use Cassette positions 5 and 6. Lower the flow rate when using liquids with low surface tension.

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Contact

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