Cellbag BioClear 11, BC11, pHOPT, DOOPT II

ID	Product Name
29090553	Cellbag BioClear 11, BC11, Basic
29091320	Cellbag BioClear 11, BC11, Basic
29091319	Cellbag BioClear 11, BC11, Basic
29091315	Cellbag BioClear 11, BC11, Basic
29091434	Cellbag BioClear 11, BC11, Basic
29097508	Cellbag BioClear 11, BC11, DO
29091341	Cellbag BioClear 11, BC11, DO
29091342	Cellbag BioClear 11, BC11, DO
29091339	Cellbag BioClear 11, BC11, DO
29091340	Cellbag BioClear 11, BC11, DO
29091348	Cellbag BioClear 11, BC11, DO, RM
29091347	Cellbag BioClear 11, BC11, DO, RM
29091352	Cellbag BioClear 11, BC11, DO, RM
29097510	Cellbag BioClear 11, BC11, DO, RM
29091353	Cellbag BioClear 11, BC11, DO, RM
29176994	Cellbag BioClear 11, BC11, Perf, DO
29091346	Cellbag BioClear 11, BC11, Perf, DO
29091344	Cellbag BioClear 11, BC11, Perf, DO
29091343	Cellbag BioClear 11, BC11, Perf, DO
29099162	Cellbag BioClear 11, BC11, pHOPT, DO
29099138	Cellbag BioClear 11, BC11, pHOPT, DO
29097512	Cellbag BioClear 11, BC11, pHOPT, DO
29097509	Cellbag BioClear 11, BC11, pHOPT, DO
29097447	Cellbag BioClear 11, BC11, pHOPT, DO
29097505	Cellbag BioClear 11, BC11, pHOPT, DO
29097502	Cellbag BioClear 11, BC11, pHOPT, DO
29099894	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29099159	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29097511	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29097516	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29097452	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29097506	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29097503	Cellbag BioClear 11, BC11, pHOPT, DOOPT II
29091332	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Perfusion
29091329	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Perfusion
29091331	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Perfusion
29091330	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Perfusion
29097453	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Screwcap
29097504	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Screwcap
29097507	Cellbag BioClear 11, BC11, pHOPT, DOOPT II and Screwcap
29091354	Cellbag BioClear 11, BC11, Screw, DO
29091357	Cellbag BioClear 11, BC11, Screw, DO
29091355	Cellbag BioClear 11, BC11, Screw, DO

Troubleshooting
Accessories

Accessories

Accessories for Cellbag DOOPT II and pHOPT version CB0010L10-31 and Cellbag, 10L, BC11, pHOPT, DOOPT II CB0010L11-31

Port No.	Description	Associated products
1 and 2	1/4 in × 7/16 in × 39 in C-Flex®, ReadyMate	ReadyMate DAC 250 with 1/4 in barb 28936691 (50 pieces) and 28936612 (10 pieces) ReadyMate DAC 375 with 3/8 in barb 28936692 (50 pieces) and 28936687 (10 pieces) ReadyMate DAC 500 with 1/2 in barb code no 28936693 (50 pieces) and 28936688 (10 pieces) ReadyMate DAC 750 with 3/4 in barb 28936694 (50 pieces) and 28936689 (10 pieces) ReadyMate DAC 750 Mini TC 28936707 (50 pieces) and 28936695 (10 pieces) ReadyMate DAC 1500 TC 28956890 (50 pieces) and 28956889 (10 pieces) ReadyClamp 28936690 (25 pieces)
3	3/16 in × 3/8 in × 2 in silicone, needleless sampling	Syringe with Luer male connection.
4	1/8 in × ¼ in × 2 in C-Flex®, y-connector 1/8 in × ¼ in × 18 in C-Flex®, Luer female	Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001)
5	1/4 in × 7/16 in × 39 in C-Flex®, MPC female	Connector MPC male and tubing i.d. 1/4 in
6	Air inlet filter	Tubing to connect inlet filter to system supplied with system
7	Air outlet filter, check valve	Electrical heater for cellbag vent filter 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system.
8	Optical pH sensor embedded in bag	Bag sensor adaptor 28984189
9	Optical DO sensor embedded in bag	Bag sensor adaptor 28984189

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#	Product Name	Product Code	Price
1	ReadyMate Disposable Aseptic Connector (DAC) 250 with 1/4" Barb, 10	28936612	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 375 with 3/8" Barb, 10	28936687	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 500 with 1/2" Barb, 10	28936688	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 with 3/4" Barb, 10	28936689	495.00 USD	Add to cart
1	ReadyClamp	28936690	574.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 250 with 1/4" Barb, 50	28936691	2,038.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 375 with 3/8" Barb, 50	28936692	2,059.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 500 with 1/2" Barb, 50	28936693	2,059.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 with 3/4" Barb, 50	28936694	2,041.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 Mini TC, 10	28936695	547.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 Mini TC, 50	28936707	2,254.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 1500 TC, 10	28956889	593.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 1500 TC, 50	28956890	2,494.00 USD	Add to cart
7	Electric Exhaust Filter Heater	28411639	548.00 USD	Add to cart
8	Bag Sensor Adaptor 2.5m Assy	28984189	1,289.00 USD	Add to cart
9	Bag Sensor Adaptor 2.5m Assy	28984189	1,289.00 USD	Add to cart

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Troubleshooting for Cellbags

The Cellbag appears to be overinflated

Possible cause	Suggested remedy
Too high airflow	Check that the airflow to the bioreactor does not exceed 0.5 Lpm. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)
Faulty relief valve	Check that air is passing out of the pressure relief valve: attach a short length of tubing to the exhaust vent and immerse the tubing in water to a 1 cm depth. Bubbles should appear indicating flow. If no flow is observed, remove the pressure relief valve. The outlet filter may be plugged and removing the pressure relief valve may allow continued operation. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)
Faulty Cellbag	If the Cellbag continues to overinflate, transfer the contents to another Cellbag. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Possible cause

Suggested remedy

Too high airflow

Check that the airflow to the bioreactor does not exceed 0.5 Lpm.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Faulty relief valve

Check that air is passing out of the pressure relief valve: attach a short length of tubing to the exhaust vent and immerse the tubing in water to a 1 cm depth. Bubbles should appear indicating flow. If no flow is observed, remove the pressure relief valve. The outlet filter may be plugged and removing the pressure relief valve may allow continued operation.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

Faulty Cellbag

If the Cellbag continues to overinflate, transfer the contents to another Cellbag.

(The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)

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The Cellbag appears to be underinflated. (An underinflated Cellbag will generate excessive foam and poor mixing.)

Possible cause	Suggested remedy
Too low airflow	Check that there is sufficient air flow to the inlet filter. (An underinflated Cellbag will generate excessive foam and poor mixing.)
Inlet air supply wrongly connected	Check that you have connected the inlet air supply line to the inlet filter.
Missing pressure relief valve	Check that the pressure relief valve is present on the exhaust filter.
Obstructed flow paths	Check that air inlet and out flow paths are unobstructed.
Clogged exhaust filter	If the exhaust filter is clogged then transfer the contents to another Cellbag.

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Excessive foaming in the Cellbag. Some amount of foam is typical. Foam should not cover more than 50% of the surface area.

Possible cause	Suggested remedy
Poorly inflated Cellbag	Check that the Cellbag is rigidly inflated. A poorly inflated bag will foam rapidly.
Too high rocking rate	If foam is still excessive after a few hours of operation, reduce the rocking rate. Make sure that the new rocking rate provides a sufficient dissolved oxygen concentration.
Too big rock angle	Reduce the rock angle. The bioreactor base units are factory set to ±6° from the horizontal. This angle has been found optimal for most cell lines.

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Contact

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