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There are different digital imaging technologies available for Western blot protein detection. How do you choose between using a CCD camera and a laser scanner?Continue reading
The polyhistidine-tag is the most widely used affinity tag in research involving recombinant proteins. It is small, and hence, the first choice in many protein purification workflows. Purification of his-tagged proteins is often straightforward using an immobilized metal ion affinity chromatography (IMAC) resin. Still, low or no recovery of your target protein can occur.
His-tagged protein purification is popular, but doing it well is a bit of an art. I recently attended a colleague’s webinar and wanted to share some of her tips for a successful outcome. I also recommend that you listen to her presentation and the live question and answer session.