探索单细胞蛋白组学的世界

聚焦出版物

出版物和演讲稿

 

聚焦出版物

1. 表观遗传学：RNA 加工

本研究中，使用西兰花荧光 RNA 适配体作为报告基因，通过凝胶荧光检测使其表达可视化。通过 Amersham Typhoon 5 生物分子成像仪检测凝胶中与西兰花结合的 3,5-二氟-4-羟基苯并咪唑啉酮 (DFHBI) 的荧光信号，并使之可视化。总 RNA 用溴化乙锭或 SYBR™ Gold 染色，然后在 Amersham Imager 600 上成像，随后使用 ImageQuant TL 软件进行分析。进行了放射性同位素标记的 Northern 印迹实验，并通过 Amersham Typhoon 系统的磷屏成像模式捕获图像。

Schmidt CA，Giusto JD，Bao A，et al. Molecular determinants of metazoan tricRNA biogenesis. Nucleic Acids Res. 2019;47(12):6452-6465.https://doi.org/10.1093/nar/gkz311

参考文献列表 ID 号：2

2. 细胞生物学：干细胞

在本研究中，使用 Amersham Typhoon 5 生物分子成像仪以分辨率为 10 µm 在结肠全组织切片中检测了红色荧光 tdTomato (tdTom) 报告基因的表达。通过这种全组织方法，可以清晰地观察和呈现 2 × 2 mm 范围内的 tdTom 阳性结肠隐窝组织。使用该报告系统使 Atoh1 表达细胞中的转录因子 Atoh1 可视化，并阐明这些细胞对上皮再生的贡献。

Tomic G, Morrissey E, Kozar S, et al. Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration. Cell Stem Cell. 2018;23(3):436-443.e7. https://doi.org/10.1016/j.stem.2018.07.002

参考文献列表 ID 号：7

3. 基因工程：植物

在本研究中，利用 Amersham Typhoon RBG 生物分子成像仪对转质体拟南芥植物中的黄色荧光蛋白 (YFP) 报告基因和叶绿素的表达进行了可视化观察。转质体幼苗在培养皿中生长。分别使用 488 nm 和 635 nm 激发共同检测 YFP 和叶绿素荧光信号。对装有样本的整个培养皿进行扫描并捕获图像。证实转质体拟南芥整个幼苗中报告蛋白的表达和叶绿素含量均较高。

Ruf, S., Forner, J., Hasse, C. et al. High-efficiency generation of fertile transplastomic Arabidopsis plants. Nat. Plants 5, 282–289 (2019). https://doi.org/10.1038/s41477-019-0359-2

参考文献列表 ID 号：19

4. 细胞生物学：酵母

在本研究中，使用 Cy5 偶联二抗对酵母细胞膜组分进行了荧光 Western 印迹分析。印迹膜图像通过 Amersham Typhoon 成像仪捕获。通过该 Western 印迹法，作者展示了磷脂合成酶的表达水平。随后采用薄层层析分离法对磷脂进行表征。用放射性同位素标记磷脂，并使用磷屏和 Amersham Typhoon 5 型号捕获图像。

Kudo S, Shiino H, Furuta S, Tamura Y. Yeast transformation stress, together with loss of Pah1, phosphatidic acid phosphatase, leads to Ty1 retrotransposon insertion into the INO4 gene. FASEB J. 2020;34(3):4749-4763. https://doi.org/10.1096/fj.201901811RR

参考文献列表 ID 号：29

5. PET 成像：新 PET 配基开发

在本研究中，使用磷屏和 Amersham Typhoon 5 荧光成像模式对新开发的 PET 配基（大鼠和非人灵长类动物 [NHP] 脑组织切片中¹⁸F 标记的 N-甲基-D-天冬氨酸受体 [NMDAR] 强效拮抗剂）进行了体外放射自显影。与¹⁸F 标记的 PET 配基孵育后，在来自大鼠和 NHP 的脑冷冻切片中识别了新 PET 配基的定位。

Fu H, Tang W, Chen Z, et al. Synthesis and Preliminary Evaluations of a Triazole-Cored Antagonist as a PET Imaging Probe ([¹⁸F]N2B-0518) for GluN2B Subunit in the Brain. ACS Chem Neurosci. 2019;10(5):2263-2275. https://doi.org/10.1021/acschemneuro.8b00591

参考文献列表 ID 号：11

出版物和演讲稿

出版物和演讲稿

1. Siddiquey MNA, Zhang H, Nguyen CC, et al. The Human Cytomegalovirus Endoplasmic Reticulum-Resident Glycoprotein UL148 Activates the Unfolded Protein Response. J Virol. 2018;92(20):e00896-18. Published 2018 Sep 26. https://doi.org/10.1128/JVI.00896-18
2. Holder JP, Izumi N, Beach M, et al. On the system stability and calibration of the image plate/scanner system for plasma diagnosis at the National Ignition Facility. Rev Sci Instrum. 2018;89(10):10F123. https://doi.org/10.1063/1.5039363
3. Tombak EM, Männik A, Burk RD, et al. The molecular biology and HPV drug responsiveness of cynomolgus macaque papillomaviruses support their use in the development of a relevant in vivo model for antiviral drug testing. PLoS One. 2019;14(1):e0211235. Published 2019 Jan 25. https://doi.org/10.1371/journal.pone.0211235

细胞生物学

1. Ueda E, Tamura Y, Sakaue H, et al. Myristoyl group-aided protein import into the mitochondrial intermembrane space. Sci Rep. 2019;9:1185. https://doi.org/10.1038/s41598-018-38016-1
2. Tomic G, Morrissey E, Kozar S, et al. Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration. Cell Stem Cell. 2018;23(3):436-443.e7. https://doi.org/10.1016/j.stem.2018.07.002
3. Ruf, S., Forner, J., Hasse, C. et al. High-efficiency generation of fertile transplastomic Arabidopsis plants. Nat. Plants 5, 282–289 (2019). https://doi.org/10.1038/s41477-019-0359-2
4. Fechter P. Mapping Changes in Cell Surface Protein Expression Through Selective Labeling of Live Cells. Methods Mol Biol. 2018;1737:119-127. https://doi.org/10.1007/978-1-4939-7634-8_8

基因组学

1. Lyon SE, Chen TH, Wallace AJ, Adib K, Gopalan V. An RNase P-Based Assay for Accurate Determination of the 5'-Deoxy-5'-azidoguanosine-Modified Fraction of in Vitro-Transcribed RNAs. Chembiochem. 2018;19(22):2353-2359. https://doi.org/10.1002/cbic.201800447
2. Schmidt CA, Giusto JD, Bao A, et al. Molecular determinants of metazoan tricRNA biogenesis. Nucleic Acids Res. 2019;47(12):6452-6465. https://doi.org/10.1093/nar/gkz311
3. Saha R, Verbanic S, Chen IA. Lipid vesicles chaperone an encapsulated RNA aptamer. Nat Commun. 2018;9(1):2313. Published 2018 Jun 13. https://doi.org/10.1038/s41467-018-04783-8
4. Beránek V, Reinkemeier CD, Zhang MS, Liang AD, Kym G, Chin JW. Genetically Encoded Protein Phosphorylation in Mammalian Cells. Cell Chem Biol. 2018;25(9):1067-1074.e5. https://doi.org/10.1016/j.chembiol.2018.05.013
5. Matera AG, Raimer AC, Schmidt CA, et al. Composition of the Survival Motor Neuron (SMN) Complex in Drosophila melanogaster. G3 (Bethesda). 2019;9(2):491-503. Published 2019 Feb 7. https://doi.org/10.1534/g3.118.200874
6. Vještica A, Merlini L, Nkosi PJ, Martin SG. Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization. Nature. 2018;560(7718):397-400. https://doi.org/10.1038/s41586-018-0407-5
7. Klein HL, Ang KKH, Arkin MR, et al. Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes. Microb Cell. 2019;6(1):65-101. Published 2019 Jan 7. https://doi.org/10.15698/mic2019.01.665
8. Elbarbary RA, Maquat LE. Evaluating the susceptibility of AGO2-loaded microRNAs to degradation by nucleases in vitro. Methods. 2019;152:18-22. https://doi.org/10.1016/j.ymeth.2018.05.010
9. Knutson SD, Arthur RA, Johnston HR, et al. Selective Enrichment of A-to-I Edited Transcripts from Cellular RNA Using Endonuclease V. J Am Chem Soc. 2020;142(11):5241-5251. https://doi.org/10.1021/jacs.9b13406
10. Derbis M, Konieczny P, Walczak A, et al. Quantitative Evaluation of Toxic Polyglycine Biosynthesis and Aggregation in Cell Models Expressing Expanded CGG Repeats. Front Genet. 2018;9:216. Published 2018 Jun 19. https://doi.org/10.3389/fgene.2018.00216
11. Walton T, Pazienza L, Szostak JW. Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension. Biochemistry. 2019;58(6):755-762. https://doi.org/10.1021/acs.biochem.8b01156
12. Taylor K, Sznajder LJ, Cywoniuk P, et al. MBNL splicing activity depends on RNA binding site structural context. Nucleic Acids Res. 2018;46(17):9119-9133. https://doi.org/10.1093/nar/gky565
13. Kroupova A, Ivascu A, Reimão-Pinto MM, Ameres SL, Jinek M. Structural basis for acceptor RNA substrate selectivity of the 3' terminal uridylyl transferase Tailor. Nucleic Acids Res. 2019;47(2):1030-1042. https://doi.org/10.1093/nar/gky1164
14. Iwasaki S, Iwasaki W, Takahashi M, et al. The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol Cell. 2019;73(4):738-748.e9. https://doi.org/10.1016/j.molcel.2018.11.026
15. Ruszkowska A, Ruszkowski M, Dauter Z, et al. Structural insights into the RNA methyltransferase domain of METTL16. Sci Rep. 2018;8(1):5311. Published 2018 Mar 28. https://doi.org/10.1038/s41598-018-23608-8

PET

1. Fu H, Tang W, Chen Z, et al. Synthesis and Preliminary Evaluations of a Triazole-Cored Antagonist as a PET Imaging Probe ([¹⁸F]N2B-0518) for GluN2B Subunit in the Brain. ACS Chem Neurosci. 2019;10(5):2263-2275. https://doi.org/10.1021/acschemneuro.8b00591
2. ULindemann M, Hinz S, Deuther-Conrad W, et al. Radiosynthesis and in vivo evaluation of a fluorine-18 labeled pyrazine based radioligand for PET imaging of the adenosine A_2B receptor. Bioorg Med Chem. 2018;26(16):4650-4663. https://doi.org/10.1016/j.bmc.2018.07.045

蛋白质研究

1. Zemella A, Thoring L, Hoffmeister C, et al. Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin. Sci Rep. 2018;8(1):8514. Published 2018 Jun 4. https://doi.org/10.1038/s41598-018-26936-x
2. Ordureau A, Paulo JA, Zhang W, et al. Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics. Mol Cell. 2018;70(2):211-227.e8. https://doi.org/10.1016/j.molcel.2018.03.012