Sensor Chip SA is a high-affinity streptavidin-coated sensor chip designed for the immobilization of biotinylated molecules. It is used in surface plasmon resonance (SPR) interaction analysis on Biacore™ systems to study biomolecular interactions in real-time without the need for labeling.

Sensor Chip SA is specifically optimized for biotinylated ligands, including proteins, peptides, nucleic acids, and small molecules. The strong and specific biotin-streptavidin interaction ensures stable and oriented immobilization, which is ideal for high-sensitivity kinetic and affinity studies.

Sensor Chip SA is not designed for reversible ligand capture. Once a biotinylated ligand is bound to the streptavidin surface, it is permanently immobilized. Therefore, the chip is typically used for single-ligand assays or until the surface is saturated. For reversible capture, Cytiva suggests using Sensor Chip CAP with the Biotin CAPture Kit.

Sensor Chip SA offers:

• High binding affinity and specificity for biotinylated ligands.
• Stable immobilization, ideal for long kinetic runs or multiple analyte injections.
• Low non-specific binding, which improves data quality.
• Compatibility with a wide range of Biacore™ systems and applications, including small molecule and fragment screening.

Choose Sensor Chip SA when:

• You need permanent immobilization of a biotinylated ligand.
• You are performing long-term or repeated analyses with the same ligand.
• Choose Sensor Chip CAP when:
• You require reversible capture of ligands.
• You are working with multiple ligands in the same assay or need to regenerate the surface between runs.