凝胶过滤产品_层析填料和预装柱_产品与技术_蛋白质研究_思拓凡官网

体积排除色谱选择指南

如要查找符合需求的介质或预装柱，请从以下描述中选择最符合您应用程序的选项。

体积排除色谱

要进行制备纯化或分析

只需要除盐或更换缓冲液

Product	Product Name	Pack Size
17059901	Sephacryl S-300 HR, 750 mL	1	View Product
17104301	Superdex 200 Prep Gradesize exclusion resin, 150 mL	150 mL	View Product

Product	Product Name	Pack Size
17060901	Sephacryl S-400 HR, 750 mL	1	View Product
17061301	Sephacryl S-500 HR, 750 mL	1	View Product

Product	Product Name	Pack Size
17058401	Sephacryl S-200 HR, 750 mL	1	View Product
17058410	Sephacryl S-200 HR, 150 mL	1	View Product
17061201	Sephacryl S-100 HR, 750 mL	1	View Product
17061210	Sephacryl S-100 HR, 150 mL	1	View Product
17104401	Superdex 75 Prep Grade size exclusion resin, 150 mL	150 mL	View Product

Product	Product Name	Pack Size
17090501	Superdex 30 Prep Grade size exclusion resin, 150 mL	150 mL	View Product

Product	Product Name	Pack Size
28989331	Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 16/600 column	1 × 120 mL	View Product
28989332	Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column	1 × 320 mL	View Product
29219757	Superdex 30 Increase 10/300 GL	1	View Product
29219758	Superdex 30 Increase 3.2/300	1	View Product

Product	Product Name	Pack Size
17001001	Sephadex™ G-10 resin, 100 g	100 g	View Product
17001002	Sephadex™ G-10 resin, 500 g	500 g	View Product

Product	Product Name	Pack Size
28918010	PD MiniTrap G-10	50 columns	View Product
28918011	PD MidiTrap G-10	50 columns	View Product

Product	Product Name	Pack Size
17059901	Sephacryl S-300 HR, 750 mL	1	View Product
17104301	Superdex 200 Prep Gradesize exclusion resin, 150 mL	150 mL	View Product

Product	Product Name	Pack Size
17116701	HiPrep 16/60 Sephacryl S-300 HR column, 16 mm × 600 mm	120 ml	View Product
17119601	HiPrep 26/60 Sephacryl S-300 HR column, 26 mm × 600 mm	320 ml	View Product
28989335	Superdex 200 pg in HiLoad column, 16/600	1 × 120 mL	View Product
28989336	Superdex 200 pg in HiLoad column, 26/600	1 × 320 mL	View Product
28990944	Superdex 200 Increase SEC column, 10/300 GL	1	View Product
28990945	Superdex 200 Increase SEC column, 5/150 GL	1	View Product
28990946	Superdex 200 Increase SEC column, 3.2/300	1	View Product

Product	Product Name	Pack Size
17060901	Sephacryl S-400 HR, 750 mL	1	View Product
17061301	Sephacryl S-500 HR, 750 mL	1	View Product

Product	Product Name	Pack Size
17058401	Sephacryl S-200 HR, 750 mL	1	View Product
17058410	Sephacryl S-200 HR, 150 mL	1	View Product
17061201	Sephacryl S-100 HR, 750 mL	1	View Product
17061210	Sephacryl S-100 HR, 150 mL	1	View Product
17104401	Superdex 75 Prep Grade size exclusion resin, 150 mL	150 mL	View Product

Product	Product Name	Pack Size
17003301	Sephadex G-25 Medium size exclusion resin, 100 g	1	View Product
17003302	Sephadex G-25 Medium size exclusion resin, 500 g	1	View Product

Product	Product Name	Pack Size
11000329	HiTrap Desalting, 100 x 5 ml	100 x 5 mL
17085101	PD-10 desalting column, with Sephadex™ G-25 resin, 1.0–2.5 mL samples (box of 30)	30 columns	View Product
17140801	HiTrap Desalting, 5 x 5 ml	5 × 5 mL	View Product
28918004	PD SpinTrap G-25	50 columns	View Product
28918006	PD MultiTrap G-25	4	View Product
28918007	PD MiniTrap G-25	50 columns	View Product
28918008	PD MidiTrap G-25	50 columns	View Product
29048684	HiTrap Desalting, 1 x 5 ml	1 × 5 mL	View Product

Product	Product Name	Pack Size
17003101	Sephadex G-25 Superfine size exclusion resin, 100 g	1	View Product
17003102	Sephadex G-25 Superfine size exclusion resin, 500 g	1	View Product
17003201	Sephadex G-25 Fine size exclusion resin, 100 g	1	View Product
17003202	Sephadex G-25 Fine size exclusion resin, 500 g	1	View Product

Product	Product Name	Pack Size
11000329	HiTrap Desalting, 100 x 5 ml	100 x 5 mL
17140801	HiTrap Desalting, 5 x 5 ml	5 × 5 mL	View Product
17508701	HiPrep 26/10 desalting column, 1/pack	1	View Product
17508702	HiPrep 26/10 desalting column, 4/pack	4	View Product
29048684	HiTrap Desalting, 1 x 5 ml	1 × 5 mL	View Product

在线选择工具将指导您选择适合预期用途的产品。在某些情况下，合适的产品可能不止一种，需要从具有不同分离范围的产品中进行选择。

凝胶过滤 (SEC) 介质的分离范围决定了可以分离出的分子量范围。一般的选择原则为，选择目标蛋白的分子量处于分离范围中间附近的介质。但当有多个合适的选项时，请考虑最贴合自己的需求的产品。例如，其中某一可选产品的分离范围可能更宽，可以更好地分离目标蛋白与特定污染物。或者，某一产品可能具有更高的流速。

$fractionation ranges$

Cytiva 提供了四类体积排阻色谱 (SEC) 介质，其分辨率的高低顺序如下：Superdex > Superose > Sephacryl > Sephadex。有多种产品可供选择，包括用于层析系统的预填充柱、用于人工纯化的预填充规格以及散装填料。

Sephadex 用于组分离，如脱盐和缓冲液更换。Sephadex LH-20 介质可用于有机溶剂，例如用于中药 (TCM)。其他介质用于高分辨率分离，以确认蛋白质纯度或进一步纯化经部分纯化或几乎纯净的蛋白质。

Superose 制备级介质的分离范围广泛，而 Sephacryl 和 Superdex 制备级介质适用于在实验室和工业范围进行快速、高回收率的分离，其中 Superdex 的分辨率最高。

Superdex Increase 和 Superose Increase 是下一代琼脂糖 SEC 介质，适用于小型制备纯化和分析（样本体积达 500 μl）。由于基架更小、更坚固，其在提高分辨率和缩短运行时间方面有所提升。他们最终将取代上一代 Superdex 和 Superose。

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