作者：王新泉，张林琦，张政等
发表年份：2020年
发表期刊：Nature
作者单位：清华大学、深圳三院等
地区：北京市，深圳市
文章摘要
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a global health emergency that is in urgent need of intervention. The entry of SARS-CoV-2 into its target cells depends on binding between the receptor-binding domain (RBD) of the viral spike protein and its cellular receptor, angiotensin-converting enzyme 2 (ACE2). Here we report the isolation and characterization of 206 RBD-specific monoclonal antibodies derived from single B cells from 8 individuals infected with SARS-CoV-2. We identified antibodies that potently neutralize SARS-CoV-2; this activity correlates with competition with ACE2 for binding to RBD. Unexpectedly, the anti-SARS-CoV-2 antibodies and the infected plasma did not cross-react with the RBDs of SARS-CoV or Middle East respiratory syndrome-related coronavirus (MERS-CoV), although there was substantial plasma cross-reactivity to their trimeric spike proteins. Analysis of the crystal structure of RBD-bound antibody revealed that steric hindrance inhibits viral engagement with ACE2, thereby blocking viral entry. These findings suggest that anti-RBD antibodies are largely viral-species-specific inhibitors. The antibodies identified here may be candidates for development of clinical interventions against SARS-CoV-2.
实验方法
使用机型：Biacore T200
实验类型：亲和力/动力学表征，表位分析，竞争性结合
样品类型：配体：RBDs 蛋白；分析物：monoclonal antibodies
实验芯片：S系列CM5芯片
配体固定方式：氨基偶联
运行缓冲液：HEPES
产品信息
| 类别 | 货号 | 产品名称 | 规格 |
|---|---|---|---|
| 主机 | Biacore T200 | ||
| 芯片 | 29104988 | S系列CM5芯片 | 1片 |
| 芯片 | BR100530 | S系列CM5芯片 | 3片 |
| 芯片 | 29149603 | S系列CM5芯片 | 10片 |
| 试剂盒 | BR100050 | 氨基偶联试剂盒 | |
| 试剂 | BR100351 | 10mM醋酸钠（PH 5.0） | 1 × 50 mL |
| 运行缓冲液 | BR100669 | HBS-EP+ (10×) | 1 × 1000 mL |
| 运行缓冲液 | BR100826 | HBS-EP+ (10×) | 4 × 50 mL |
Reference
Ju B, Zhang Q, Ge J, Wang R, Sun J, Ge X, Yu J, Shan S, Zhou B, Song S, Tang X, Yu J, Lan J, Yuan J, Wang H, Zhao J, Zhang S, Wang Y, Shi X, Liu L, Zhao J, Wang X, Zhang Z, Zhang L. Human neutralizing antibodies elicited by SARS-CoV-2 infection. Nature. 2020 Aug;584(7819):115-119. doi: 10.1038/s41586-020-2380-z. Epub 2020 May 26. PMID: 32454513.