FAQ
No, ensure that no magnetic particles are carried over into the PCR reaction as this may affect downstream performance.
Yes, Sera-Mag Select is manufactured to standards suitable for both PCR clean-up and size selection. Protocols can be found in the User Guide at cytivalifesciences.com/shop/molecular-biology/pcr-and-amplification/kits-and-ready-to-go-beads/sera-mag-select-p-10218.
The effective elimination of both primers and adaptors from the PCR can be accomplished by following the size selection protocol in the User Guide at cytivalifesciences.com/shop/molecular-biology/pcr-and-amplification/kits-and-ready-to-go-beads/sera-mag-select-p-10218.
The smallest fragment size we would recommend for size selection using Sera-Mag Select is 100 bp. Please refer to the size selection protocol, found in the User Guide at cytivalifesciences.com/shop/molecular-biology/pcr-and-amplification/kits-and-ready-to-go-beads/sera-mag-select-p-10218.
Sera-Mag Select will bind genomic DNA, but it is not designed for this purpose. Extremely large DNA strands can prove difficult to elute and may bind across magnetic particles causing them to aggregate.
For the majority of applications, Sera-Mag Select may be substituted directly for AMPure XP without any change to the existing script(s), though we recommend that the user check this before substituting Sera-Mag Select into the workflow.
Selecting fragments in a wider range can reduce sequencing efficiency. Note that for a given amount of starting material, selection of a tighter size range from a fragmented genomic DNA sample will reduce overall yield, but will have a higher percentage of usable fragments. This can improve the amount of usable data from the sequencing run.