HiTrap DEAE Sepharose FF

Prepacked column for small-scale purification using weak anion exchange resin

HiTrap™ DEAE Sepharose™ Fast Flow prepacked column for weak anion exchange in protein purification workflows

Several bottles of Sepharose resins and Sepharose columns of various sizes

HiTrap DEAE Sepharose FF

Prepacked column for small-scale purification using weak anion exchange resin

包装规格

5 × 1 mL

5 × 5 mL

如需购买，请选择您的产品

Carbon neutral shipping included

概述

HiTrap DEAE FF 预填充了 DEAE Sepharose FF 填料，是一种用于小规模蛋白纯化以及筛选结合和洗脱条件的弱阴离子交换介质。

便捷且经济实惠，可按单独或串联形式进行快速、简便的离子交换分离。
离子交换层析的行业标准。
高流速，优良的放大性。
如果强离子交换介质的选择性不足，可以尝试使用弱离子交换介质。
可对放大规模进行预测

Q、SP、DEAE 和 CM Sepharose FF 填料基于可靠的 6% 高度交联的琼脂糖基质，具有良好的流速特性和高结合载量。ANX Sepharose 4 FF 填料（高取代）基于 4% 高度交联的琼脂糖。这产生具有更高孔隙率的填料，有利于高分子量蛋白质的纯化。

DEAE Sepharose FF 填料和 ANX Sepharose FF 填料（高取代）的带电荷基团的活性端相同，差异为带电荷基团的碳链长度。DEAE Sepharose FF 填料是琼脂糖基质结合了二乙氨乙基，而 ANX Sepharose 4 FF 填料（高取代）结合的是二乙基氨丙基。

产品规格

共 2 个产品规格

17505501

缺货

询价

17515401

缺货

询价

产品供应无法保证，且可能随时变更。

产品支持

常见问题

What is DEAE Sepharose resin used for in protein purification?

DEAE Sepharose™ Fast Flow resin is a weak anion exchanger used for small-scale protein purifications and screening of binding/elution conditions.

How does HiTrap DEAE Sepharose FF column work?

HiTrap™ DEAE FF column arrives prepacked with Sepharose™ resin, which is based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacity. HiTrap™ DEAE Sepharose™ FF column is used to perform ion exchange to fractionate proteins and peptides, even those with only a small difference in charge.

Can HiTrap columns be used with FPLC systems?

Yes, HiTrap™ columns can be used with FPLC systems. ÄKTA™ chromatography systems include preset method templates for HiTrap™ prepacked columns, which simplifies operation, improves reproducibility, and saves time.

What is the difference between DEAE and Q Sepharose resin?

DEAE Sepharose™ resin is a weak anion exchange resin. Q Sepharose™ resin is a strong anion exchange resin. The binding capacity of weak ion exchangers varies considerably more with pH than strong ion exchangers, which might affect selectivity. The ligands of strong ion exchangers remain charged, with a consistently high capacity maintained over a broad working pH range.

安全数据表搜索

搜索安全数据表文件。

证书搜索

搜索证书分析文件(CoA)，质量标准(CoQ)，符合性证书(CoC)

资源中心

查看最实用的在线工具、最新的文章、产品手册等资源。

联系我们

客户服务、订单、科学技术问题