Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

Capto S ImpAct resin is available in bulk container formats and in prepacked chromatography formats, to support various scales, from screening to GMP manufacturing. process development.

Typical chromatogram demonstrating capabilities for aggregate and host cell protein removal at high load (64 mg/mL) with linear salt gradient elution.

Schematic picture of the polymer-grafted Capto™ S ImpAct resin. The polymer surface extender is formed by random grafting between the two building blocks

Typical mAb purification process with a protein A affinity capture step, followed by two polishing steps

Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

包装规格

1 L

5 L

10 L

25 mL

60 L

100 mL

Capto S ImpAct 填料

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Carbon neutral shipping included

概述

Capto S ImpAct 具有高结合载量和小粒径，对于工业纯化工艺中可靠、稳定的精纯步骤而言是一种理想选择。

高结合载量，> 100 mg mAb/mL 填料。
在高单克隆抗体上样量的条件下实现高效聚集体去除。
基于成熟的 Capto 平台的高分辨率精纯。
由于流速和柱床高度操作区间大，设计灵活，易于优化和放大。
高生产效率使生产经济高效。

设计用于大进料体积

该层析填料是基于 Capto 产品线的高分辨率填料平台的组成部分。聚合配基结合基质的属性，使该阳离子交换介质具有高结合载量。此外，小粒径填料可实现高分辨率纯化。

能够在高流速下运行

Capto 填料的高流速特性提供了良好的压力流速特性。Capto S ImpAct 的高结合载量和分辨率结合可在较高流速和较高柱床高度下运行的能力，提高了工艺设计的生产效率和灵活性。

适用于工业规模的应用

Capto S ImpAct 是一种 BioProcess 层析填料，开发用于生物制药的大规模纯化。基于成熟的 Capto 基质，该填料为提高生产效率和改善工艺开发流程提供了许多机会。

预装柱和其他规格

Capto S ImpAct 填料具有以下规格：

工艺开发规模：预装柱

PreDictor Capto S ImpAct 滤板（96 孔板），用于高通量筛选或工艺开发。
PreDictor Capto S ImpAct RoboColumn™ 单元是个小型化层析柱，用于高通量工艺开发。
HiScreen Capto S ImpAct 层析柱，优化用于方法和工艺开发。

实验室规模：用于手动纯化的预装柱

HiTrap Capto S ImpAct 层析柱，易于与注射器、蠕动泵或层析系统一同使用。

实验室规模：用于自动纯化和分析的预装柱

HiTrap Capto S ImpAct 层析柱，易于与注射器、蠕动泵或层析系统一同使用。

工艺表征：填料试剂盒

工艺表征试剂盒 Capto S ImpAct，设计用于研究填料配基密度对工艺结果的潜在影响。

生产规模纯化：预填充

ReadyToProcess Capto S ImpAct 层析柱，用于临床生产的 1 L 或更大层析柱，可最大程度地减少清洁和清洁验证的需要。

产品规格

共 6 个产品规格

17371701

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询价

17371702

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17371703

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17371704

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17371705

缺货

询价

17371760

缺货

询价

产品供应无法保证，且可能随时变更。

产品支持

常见问题

Ligand

Question	Answer
What is the advantage with a polymeric ligand design?	With the polymeric design, the ligand includes both spacers and functional groups. This increases the binding capacity without compromising the resolution.

Performance

Question	Answer
Is there any data showing Capto™ S ImpAct resin used as the second step in a mAb purification process?	Application note CY13612 Polishing of monoclonal antibodies using Capto™ S ImpAct includes an example of a chromatography step with Capto™ S ImpAct resin. The data file CY11840 includes dynamic binding capacity (DBC), application examples, and links to additional application notes.
How to optimize Capto™ S ImpAct resin?	For screening of a wide range of buffer conditions we recommend prepacked 96-well filter PreDictor™ plates or PreDictor™ RoboColumn, prepacked miniaturized columns. Further optimization is preferably done in laboratory-scale columns before scale-up. An example is given in CY13646.

Comparison with Capto™ S and Capto™ SP ImpRes resins

Question	Answer
What are the design and performance differences between Capto™ S ImpAct, Capto™ SP ImpRes, and Capto™ S resins?	Capto™ S ImpAct resin is a polishing resin with surface-extender technology for large molecules (mAbs) and for high capacity in combination with high resolution (even at high load). Capto™ SP ImpRes resin is a polishing resin suitable for smaller proteins that require high resolution. Capto™ S resin is a capture resin for high capacity and good pressure/flow properties. All three resins are comparable in terms of ligand leakage, alkali stability, and acidic stability.
Can the same buffer systems be used for binding and elution with Capto™ S, Capto™ SP ImpRes, and Capto™ S ImpAct resins?	Yes, the same buffer systems can be used for Capto™ S ImpAct as for Capto™ S and Capto™ SP ImpRes resins. Capto™ S ImpAct is a cation exchange (CIEX) resin and can be used as any other CIEX resin.
Capto™ S resin had some nontraditional behavior. Could similar behavior be expected with Capto™ S ImpAct resin?	Not to the same extent, but in some cases, yes. We recommend screening of pH and salt conditions to evaluate each protein that is going to be purified.

Question

Answer

What are the design and performance differences between Capto™ S ImpAct, Capto™ SP ImpRes, and Capto™ S resins?

Capto™ S ImpAct resin is a polishing resin with surface-extender technology for large molecules (mAbs) and for high capacity in combination with high resolution (even at high load).
Capto™ SP ImpRes resin is a polishing resin suitable for smaller proteins that require high resolution.
Capto™ S resin is a capture resin for high capacity and good pressure/flow properties.

All three resins are comparable in terms of ligand leakage, alkali stability, and acidic stability.

Can the same buffer systems be used for binding and elution with Capto™ S, Capto™ SP ImpRes, and Capto™ S ImpAct resins?

Yes, the same buffer systems can be used for Capto™ S ImpAct as for Capto™ S and Capto™ SP ImpRes resins. Capto™ S ImpAct is a cation exchange (CIEX) resin and can be used as any other CIEX resin.

Capto™ S resin had some nontraditional behavior. Could similar behavior be expected with Capto™ S ImpAct resin?

Not to the same extent, but in some cases, yes. We recommend screening of pH and salt conditions to evaluate each protein that is going to be purified.

Column packing

Question	Answer
Are there available packing instructions for laboratory-scale Tricorn™ and HiScale™ columns and bioprocess columns?	Yes, large scale packing instructions are found in the article Packing large-scale chromatography columns with Capto™ S ImpAct resin. Packing of lab scale, Tricorn™ and HiScale™ columns, are included in 29092501 Capto™ S ImpAct Ion Exchange Media Instructions for Use.
What is the packing factor of Capto™ S Impact?	For a 20 cm bed in 150 mM NaCl when consolidated at 60 cm/h, the packing factor is 1.20.

Pressure

Question	Answer
What is the maximum pressure for Capto™ S Impact?	Maximum back pressure over the packed bed is 3 bar (44 psi, 0.3 MPa) for HiTrap™ columns and 4 bar (58 psi, 0.4 MPa) for HiScreen™ columns.

Cleaning and sanitization

Question	Answer
What cleaning procedures should be used for Capto™ S ImpAct resin?	The instruction for use (29092501) includes guidance on how to remove specific contaminants.
How to do sanitization of Capto™ S ImpAct resin?	Regular sanitization will hinder microbial growth and maintain a high level of hygiene in the packed column. A specific sanitization protocol should be developed according to the nature and condition of the starting material. To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 h is recommended. The clean in place (CIP) protocol for precipitated, hydrophobically bound proteins or lipoproteins sanitizes the resin effectively.

Question

Answer

What cleaning procedures should be used for Capto™ S ImpAct resin?

The instruction for use (29092501) includes guidance on how to remove specific contaminants.

How to do sanitization of Capto™ S ImpAct resin?

Regular sanitization will hinder microbial growth and maintain a high level of hygiene in the packed column. A specific sanitization protocol should be developed according to the nature and condition of the starting material.
To reduce microbial contamination in the packed column, sanitization using 0.5 to 1 M NaOH with a contact time of 1 h is recommended. The clean in place (CIP) protocol for precipitated, hydrophobically bound proteins or lipoproteins sanitizes the resin effectively.

Storage

Question	Answer
Can you provide some storage recommendations not using ethanol?	We recommend 2% benzyl alcohol with 0.2 M sodium acetate.
What is the shelf life for Capto™ S ImpAct products?	The shelf life is estimated to be 5 years for Capto™ S ImpAct resin in bulk.

Antibody purification

Question	Answer
Which resin to use in mAb and antibody variant purification?	Our recommendation is to start with our platform for antibodies. MabSelect™ PrismA➔Capto™ S ImpAct➔Capto™ Q or Mustang™ Q. However, depending on what purification challenges the process may need to be fine-tuned. To learn more about options in capture, look at the capture selection guide. You can read more about polishing in this article and in the polishing selection guide.

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Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

概述

设计用于大进料体积

能够在高流速下运行

适用于工业规模的应用

预装柱和其他规格

工艺开发规模：预装柱

实验室规模：用于手动纯化的预装柱

实验室规模：用于自动纯化和分析的预装柱

工艺表征：填料试剂盒

生产规模纯化：预填充

产品规格

产品支持

常见问题

Ligand

Performance

Comparison with Capto™ S and Capto™ SP ImpRes resins

Column packing

Pressure

Cleaning and sanitization

Storage

Antibody purification

相关产品

需要更多帮助？

Shop in your local currency and language

Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

Capto S ImpAct 离子交换层析填料

A strong cation exchanger for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules.

概述

设计用于大进料体积

能够在高流速下运行

适用于工业规模的应用

预装柱和其他规格

工艺开发规模：预装柱

实验室规模：用于手动纯化的预装柱

实验室规模：用于自动纯化和分析的预装柱

工艺表征：填料试剂盒

生产规模纯化：预填充

产品规格

相关文档

application note

产品支持

常见问题

Ligand

Performance

Comparison with Capto™ S and Capto™ SP ImpRes resins

Column packing

Pressure

Cleaning and sanitization

Storage

Antibody purification

相关产品

需要更多帮助？