Get confident: preclinical production of RNA lipid nanoparticles

About course

This interactive eLearning is designed for learners to follow along in their own lab with the preclinical workflow for RNA lipid nanoparticles (LNPs) using the NanoAssemblr Ignite or Ignite+ nanoparticle formulation systems, and GenVoy-ILM training kit.  The workflow covers N/P ratio calculation, reagent preparation, encapsulation, downstream processing, and basic LNP analytics.  

At the end of this course, you will be able to: 

- Determine the input concentration of RNA for a formulation based on the parameters of the run 

- Perform the workflow for making RNA-LNPs using the NanoAssemblr Ignite or Ignite+ and GenVoy-ILM including downstream processing, particle sizing, and encapsulation efficiency 

- Understand the principles and interpret results of dynamic light scattering (DLS) and the modified RiboGreen assay 

Completion of the fundamentals course, is required. Access to a NanoAssemblr Ignite or Ignite+ system and GenVoy-ILM training kit (not provided) are required to follow along. Other equipment essential to the workflow include: 

- DLS instrument 

- UV absorption spectrometer 

- 96-well plate reader 

- single and multichannel micropipettes 

- RiboGreen assay kit 

- nuclease-free deionized water 

Dive in and learn at your own pace with this detailed 120-minute course, divided into bite-sized topics you can access and complete on demand. 

Purchasing the course for multiple users  

Would you like to enroll several people on this course? Please send us an email stating the number of users required and we’ll send you a quote. 

Certification

You will get an online certificate after the course completion. You can view and download it in a section My Courses. With Cytiva Certificates, portions of our programs have been split into online modules, so you can earn a high quality knowledge in interactive format.