pDNA and mRNA bioprocessing: theory and practice
Course
About course
In this 4-day, hands-on course, you will get a comprehensive understanding of the mRNA workflow, starting from downstream purification of plasmid DNA (pDNA).
You will get practical experience on how to purify pDNA, starting from cell lysis to the polishing chromatography step. You will then learn how to perform an IVT reaction and investigate the impact that certain parameters have on the mRNA yield. Understand mRNA purification using Fibro™ OligodT and the effect of parameters such as salt concentration and residence time.
Participants will get to know the plasmid DNA and mRNA end-to-end workflow through classroom lectures.
At the end of this course, you will be able to:
- Understand the different unit operations in plasmid DNA and mRNA manufacturing processes
- Gain practical experience in pDNA purification, mRNA synthesis and mRNA purification
- Understand process hygiene, IVT, LNP, and scale-up considerations
- Identify key analytical methods required to assess mRNA quality
Course dates and locations:
UPPSALA, SWEDEN
APR 21-24. 2026 (EN)
SHANGHAI, CHINA
JUN 9-12, 2026 (CN)
DEC 08-11, 2026 (CN)
Who should attend?
R&D, process development, and MSAT scientists interested in deepening their knowledge about design and optimization of a mRNA process.
Review the terms and conditions for Fast Trak™ training courses here.
Review the general course information here.
Prior to attending
A basic understanding of downstream bioprocessing is recommended for this course.
Refresh your knowledge with these courses:
- Get to know: filtration for plasmid DNA
- Get to know: upstream plasmid DNA processing
- Get to know: mRNA IVT reaction
The content below will be accessible after attending the course.
